TRIS (77-86-1) is conventionally white crystal particles, and the pH value of the acid-base indicator is 7-9. It is an important organic chemical raw material and a common biological buffer. Because of its wide buffering range and good buffering effect, in current research, TRIS has been continuously expanding its application. Now there are many labels such as biological buffers, surfactants, vulcanization accelerators, and pharmaceutical intermediates.
TRIS is well known and widely used as a biological buffer. If you observe carefully, you will find that it also exists in other hidden corners. It is said that TRIS is widely used but how extensive is it? Let's take a look at the editor of Desheng!
Application of TRIS in Biological Buffer
In the field of biological buffers, TRIS buffer can be used to form the intermediate fibers of nematode nuclear laminin (using the low ionic strength of its solution), and can also be used as one of the main components of protein electrophoresis buffers; Tris can also be used with other The solutions are mixed to form a new biological buffer. Generally, hydrochloric acid is added to adjust the pH to a desired value, and then a buffer solution of the pH value can be obtained. At the same time, attention should be paid to the influence of temperature on the pKa of Tris.
Application of TRIS in eye drops
Eye drops are one of the most commonly used drug formulations for ophthalmological diseases. For many eye diseases, eye drops have a direct and rapid therapeutic effect. Due to the special anatomical and physiological characteristics of the eyes, corresponding requirements are put forward for ophthalmic preparations: the pH of normal tears is 7.2-7.4, and the pH range that normal eyes can tolerate is 5.0-9.0. The pH value of the eye drops will directly affect the eye irritation and the curative effect of the drug. When preparing eye drops, a buffer should be added to adjust the pH of the solution so that it is roughly similar to the tear fluid to reduce the impact on the eyes. Stimulus. At the same time, adjusting the pH value also needs to take into account multiple factors such as drug solubility and stability. TRIS is a commonly used pH regulator in eye drops.
Application of TRIS in nucleic acid detection
Nucleic acid testing is an important means for diagnosing the new coronavirus. What is the application of TRIS in nucleic acid testing? TRIS is an additive in the virus preservation solution, which is the core material for nucleic acid detection. TRIS can maintain the pH of the sampled sample in the virus preservation solution and act as a biological buffer. The virus and its nucleic acid are relatively sensitive to the environmental pH. (DNA, RNA) is easily hydrolyzed in acidic solution, and more stable in neutral or weak alkaline solution. Its other function is to maintain the stability of the nucleic acid released after the lysis of the sample to be extracted to avoid degradation of the nucleic acid, increase the concentration and purity of the nucleic acid, and ensure the accuracy of subsequent nucleic acid detection and analysis operations.
Application of TRIS in cosmetics
TRIS is mainly used as a neutralizer in cosmetics. Neutralizers are actually PH regulators. Everyone knows that everyone's skin is different and the suitable cosmetics are also different. The ingredients in many cosmetics are very complex. If you want a suitable pH value, you must use TRIS to adjust, so that the entire raw material composition will not change too much, so that the cosmetics can be applied more gently on the face without irritation.
Desheng Technology is a professional manufacturer of biological buffers. TRIS is the company's advantage. The product has a purity of ≥99% and a high level, which can be used in many fields. Spot supply, the average daily output is ton-level, if you are interested, please click on the official website or call for consultation.