The commonly used SYBR Green I nucleic acid dye is a non-saturated fluorescent dye, which inhibits the fluorescence of LAMP at high concentrations.
Therefore, only low concentrations can be used for LAMP. At lower concentrations, the dye molecule is not linearly related to
The linear relationship between the dye molecule and DNA synthesis is not strong at lower concentrations (see the figure on the right below), so the reproducibility of the reaction is not stable. In contrast, saturated fluorescent dyes
saturated fluorescent dyes do not inhibit the fluorescent LAMP reaction even at saturated concentrations, so saturated concentrations can be used for LAMP. When using
The linear relationship between the dye molecule and DNA synthesis is strong at saturated concentrations (see the figure on the left below), so the reproducibility of the reaction is good.
The reproducibility of the reaction is very good.
This product is a saturated fluorescent dye for LAMP with the following features: 1.
1. saturated dyes, which can be used at saturated concentrations, provide better reproducibility and higher signal-to-noise ratio. 2.
It is more resistant to hydrolysis, pyrolysis and photolysis than SYBR Green I. 3.
3. Compatible with current LAMP amplification assay instruments, no need to change any settings of the instrument, directly in the
The signal can be collected directly in the SYBR Green I channel. 4.
4. cannot penetrate into the cell, toxicity and mutagenicity is much lower than SYBR Green I. 5.
5. can be used for dye LAMP, dye RT-LAMP and other studies. 6.
6. The product is sufficient for 1000 times of LAMP or RT-LAMP amplification reaction of 20uL system.
7. This product can only be used for research.